Reagents and tools table
| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| E. coli KRX | Promega | L3002 |
| Recombinant DNA | ||
| pNIC-CTHF-M21 | Reinhard et al, 2017 | N/A |
| pJ411 | DNA TwoPointO | N/A |
| pJ411:His-TEV-HsELAC2 | DNA TwoPointO | N/A |
| pJ411::His-TEV-HsELAC2_H548A | This study | N/A |
| pJ411::His-TEV-HsELAC2_T520I | This study | N/A |
| pJ411::His-TEV-HsELAC2_F154A | This study | N/A |
| pJ411::His-TEV-HsELAC2_F154L | This study | N/A |
| pJ411::His-TEV-HsELAC2_G132R | This study | N/A |
| pJ411::His-TEV-HsELAC2_V256E_L257E | This study | N/A |
| Mitochondrial tRNAHis-Ser with 5′-T7 promoter (synthetic DNA; GBlock) | Integrated DNA Technologies | NCBI NC012920.1 nucleotides 12138-12265; Assembly GRCh38.p14) |
| Oligonucleotides and other sequence-based reagents | ||
| tRNAHis-GAG-rev | Integrated DNA Technologies | CTCGGTAAATAAGGGGTCGTAAGC |
| tRNAHis-CCA-rev | Integrated DNA Technologies | TGGGGTAAATAAGGGGTCGTAAGC |
| tRNAHis-for | Integrated DNA Technologies | CCGCGAATTAATACGACTCACTATAG |
| tRNA-Ser(AGY)-rev | Integrated DNA Technologies | TGAGAAAGCCATGTTGTTAGACATG |
| ELAC2_T520I_F | Integrated DNA Technologies | GCTGCTGGATTGTGGTGAGGGTATCTTCGGTCAACTGTGCCGCC |
| ELAC2_T520I_R | Integrated DNA Technologies | GGCGGCACAGTTGACCGAAGATACCCTCACCACAATCCAGCAGC |
| ELAC2_F154L_F | Integrated DNA Technologies | GAAATACTTGGAAGCCATTAAGATCCTGTCCGGTCCGCTGAAAGGCATC |
| ELAC2_F154L_R | Integrated DNA Technologies | GATGCCTTTCAGCGGACCGGACAGGATCTTAATGGCTTCCAAGTATTTC |
| ELAC2_F154A_F | Integrated DNA Technologies | GAAATACTTGGAAGCCATTAAGATCGCGTCCGGTCCGCTGAAAGGCATC |
| ELAC2_F154A_R | Integrated DNA Technologies | GATGCCTTTCAGCGGACCGGACGCGATCTTAATGGCTTCCAAGTATTTC |
| ELAC2_G132R_F | Integrated DNA Technologies | GATTCTGACCCTGAAAGAAACCCGTCTGCCGAAGTGCGTGCTGAGC |
| ELAC2_G132R_R | Integrated DNA Technologies | GCTCAGCACGCACTTCGGCAGACGGGTTTCTTTCAGGGTCAGAATC |
| ELAC2_VL256EE_F | Integrated DNA Technologies | GCACCTGAAACGCGGCAATTTTCTGGAAGAAAAAGCGAAAGAGATGGGTCTGCCGG |
| ELAC2_VL256EE_R | Integrated DNA Technologies | CCGGCAGACCCATCTCTTTCGCTTTTTCTTCCAGAAAATTGCCGCGTTTCAGGTGC |
| Chemicals, enzymes, and other reagents | ||
| DNase I | Roche | 4536282001 |
| HiPrep™ Sephacryl™ S-300 HR | Cytiva | Cytiva 17-1167-01 |
| HiTrap® Capto™ S | Cytiva | Cytiva 29-4004-58 |
| HiTrap® Heparin High Performance | Cytiva | Cytiva 17-0406-01 |
| HiTrap® Q High Performance | Cytiva | Cytiva 17-1153-01 |
| HiTrap™ IMAC FF | Cytiva | Cytiva 17-0921-02 |
| Mono Q® 5/50 GL | Cytiva | Cytiva 17-5166-01 |
| Novex™ TBE-Urea Gels, 15% | Thermo Fisher Scientific | EC6885BOX |
| Phusion™ High-Fidelity DNA Polymerase | Thermo Fisher Scientific | F530S |
| Proteinase K Solution (20 mg/mL), RNA grade | Thermo Fisher Scientific | 25530049 |
| Superdex® 200 Increase 10/300 GL | Cytiva | Cytiva 28-9909-44, |
| SYBR™ Green II RNA Gel Stain | Thermo Fisher Scientific | S7568 |
| T7 RNA polymerase | In-house production | N/A |
| TEV protease | In-house production | N/A |
| UltrAuFoil 300 mesh R 1.2/1.3 geometry | Quantifoil Micro Tools | Q350AR13A |
| Software | ||
| AlphaFold | Jumper et al, 2021 | N/A |
| Coot | Emsley et al, 2010 | N/A |
| CryoSPARC | Punjani et al, 2017 | N/A |
| EMReady | He et al, 2023 | N/A |
| Isolde | Croll, 2018 | N/A |
| Locscale | Jakobi et al, 2017 | N/A |
| Phenix | Liebschner et al, 2019 | N/A |
| RELION | Scheres, 2012 | N/A |
| WARP | Tegunov and Cramer, 2019 | N/A |
| Other | ||
| EMS100x glow-discharge | Electron Microscopy Sciences | N/A |
| Filter paper | Ted Pella | 47000-715 |
| K3 BioQuantum | Gatan | 1967 |
| Krios G3i | Thermo Fisher Scientific | N/A |
| Vitrobot Mk IV | Thermo Fisher Scientific | N/A |