Figure 4.

Knocking down Sntb1 increases myotube fusion. (a) Scheme of cell culture and siRNA interference within myotube after differentiation for 5 days. (b) Gene expression of protein turnover and cell differentiation within myotubes transfected by siSntb1 for 24 h compared to myotubes transfected by siControl. (c) Myotubes with more nuclei were found in myotube transfected by siSntb1 by immunofluorescent staining of myosin heavy chain (MHC) (green) and nuclei (DAPI, blue). Scale bar = 250 µm. (d) Myotube diameter and (e) fusion index were increased in myotubes transfected by siSntb1. (f) More myotube with more than 10 nuclei were found when cells that were transfected by siSntb1. (g) The differentiation index was decreased in myotubes treated with siSntb1. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. N = 6. Data were analysed by independent t‐tests.