Fig. 2.

The evaluation of digital PCR for the detection of pathogenic Leptospira infection in kidneys. Our study employed a digital PCR approach designed to target the lipl32 gene, which is specific only to pathogenic species of Leptospira. This methodology, combined with conventional and real-time qPCR, was utilized for the detection and quantification of pathogenic leptospires in the kidneys of mice subjected to experimental Leptospira infection. Negative control mice were used as a reference for noninfected status, while mice with nonpathogenic L. biflexa infection served as an infection control group. Figure was created with biorender.com (accessed on Jan 2024).