Fig. 5.

Dynamic ranges and sensitivity for digital PCR assays targeting lipl32 and fliG genes. The total copy number of each sample was determined using a digital PCR (absolute total copies detection; log values) and estimated from the Ct values obtained from real-time qPCR (data are presented as means; detailed values are provided in Table 3). The X-axis represents the 10-fold dilution series of Leptospira genomic DNA, and the numbers of copies were theoretically calculated using the copy number calculator tool on the Technology Networks server (https://www.technologynetworks.com/tn/tools/copynumbercalculator). Correlation analysis between the theoretically calculated Leptospira copies (X-axis) and the copy number measured in the dilution series using both real-time qPCR and digital PCR (Y-axis, displayed as the mean values; n ≥ 3). Pearson's correlation coefficient (r) and p value are indicated. Statistical analyses were performed using Graph-Pad Prism 6.0 software (GraphPad Software, Inc.).