Table 1.
Primer-probe sets sequence designed for detection of Leptospira species.
| Gene target | Primer/probea | Sequence (5′ to 3′)b | Amplicon size (bp)c | Leptospira target | Reference |
|---|---|---|---|---|---|
| lipl32 | lipl32_F | AAGCATTACCGCTTGTGGTG | 242 | L. interrogans | Matsui et al., 2017 |
| lipl32_R | GAACTCCCATTTCAGCGATT | ||||
| lipl32_P | FAM-AAAGCCAGGACAAGCGCCG-BHQ1 | ||||
| fliG | fliG_F | ATACTTCTGCGGGTGGTATTG | 110 | L. interrogans; L. biflexa | This study |
| fliG_R | GCAAGTTCGGGATCTTCTTCT | ||||
| fliG_P1 | FAM-AGTTGAGATTCTAAACTTAGTCG ATCGGGGAACG-BHQ1 | L. interrogans | |||
| fliG_P2 | FAM-ACGGAGAAGACCATCATCGAAGCT-BHQ1 | L. biflexa |
a
(F), (R) and (P) indicate forward and reverse primer and probe sequences, respectively.
b
the probe, labelled with a unique fluorescent reporter dye [6-carboxyfluorescein (FAM)] at the 5′-end and a 3′- Black Hole Quencher 1 (BHQ1), was designed to anneal to an internal sequence within the amplified region.
c
bp, base pairs.