Table 2.
Proportion of infected mice assessed by examining the presence of Leptospira in the kidneys.
| Target gene | Post-infection (days) | Groups | Genomic DNA extracted from kidneys |
Total RNA extracted from kidneys |
||||
|---|---|---|---|---|---|---|---|---|
| Traditional PCRa | Real-time PCRa | Digital PCRa | Traditional PCRa | Real-time PCRa | Digital PCRa | |||
| lipl32 | 7 | Without infection | 0 (0/3) | 0 (0/3) | 0 (0/3) | 0 (0/3) | 0 (0/3) | 0 (0/3) |
| L. biflexa infection | 0 (0/4) | 0 (0/4) | 0 (0/4) | 0 (0/4) | 0 (0/4) | 0 (0/4) | ||
| L. interrogans infection | 0 (0/12) | 8 (1/12) | 55 (6/11) | 0 (0/12) | 0 (0/12) | 58 (7/12) | ||
| 28 | Without infection | 0 (0/3) | 67 (2/3) | 0 (0/3) | 0 (0/3) | 0 (0/3) | 0 (0/3) | |
| L. biflexa infection | 0 (0/4) | 50 (2/4) | 0 (0/4) | 0 (0/4) | 0 (0/4) | 0 (0/4) | ||
| L. interrogans infection | 0 (0/12) | 33 (4/12) | 42 (5/12) | 50 (6/12) | 8 (1/12) | 58 (7/12) | ||
a
% of mice: Percentage (no. mice with the presence of Leptospira in the kidneys/no. total mice).