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Enhanced thermal stability of PAD with and without flavin nucleotides. Purified PAD was boiled with (⧫) and without (▴) flavin nucleotides (FMN-FAD, 25 μM) or incubated at 37°C (●) in substrateless assay buffer (50 mM Tris-HCl, 25 μM FMN, pH 8.0). Aliquots were removed at various time points and assayed for the production of citrulline as described in Materials and Methods.
