Figure 4.

Irgm1 localizes to a subset of peroxisomes in macrophages. WT BMM activated with IFN-γ for 24 h were fixed and stained with anti-Irgm1 (green) or anti-PMP70 (red) antibodies. Shown are representative confocal images; arrows mark select peroxisomes that showed Irgm1 staining. Irgm1 localization to peroxisomes was quantified in 10 cells using both manual counting and ImageJ/JACoP analysis, as indicated. Error bars indicate SD. The lower control panels show representative images of the cells stained with secondary antibodies only. The nuclei in the overlay images are stained with DAPI.