Figure 6.

PPARα agonist, fenofibrate, normalizes type I IFN signaling in Irgm1-deficient macrophages.A, WT and Irgm1^−/− BMM plated on coverslips were activated with IFN-γ for 24 h in the presence or absence of fenofibrate (50 μM). The cells were then stained with anti-PMP70 antibodies and peroxisomes were enumerated. Data points represent individual cells. Shown is representative of two studies. B–D, WT and Irgm1^−/− BMM isolated from 3 to 4 distinct mice per genotype were activated with IFN-ɣ for 24 h in the presence or absence of fenofibrate. Cells were collected and used to quantify Ccl-2, Mx-1, and Ifnb mRNA using quantitative PCR. Shown is representative of two experiments. Error bars indicate SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.