FIG. 1.
CTL-mediated lysis of peptide coated EL-4 or P815 cells. Mice were injected i.p. with individual LFn fusion proteins plus PA. After in vitro stimulation, samples were assayed for their ability to lyse 51Cr-labeled EL-4 or P815 cells coated with the cognate peptide (closed circles) or not coated (open circles). EL-4 cells (H-2b) were used as targets for cultures from C57BL/6 mice. P815 cells (H-2d) were used as targets for cultures from BALB/c mice. The cultures tested in these assays were splenocytes from individual mice immunized with LFn-NP118–126 (A), LFn-NP396–404 (B), LFn-LLO91–99-NP118–126 (C), or LFn-NP118–126-NP396–404 (D). Targeting was evaluated by 51Cr release. Threefold effector dilutions are shown. Similar levels of lysis were observed for each of three replicates.