FIG. 2.

CTL-mediated lysis of peptide coated EL-4 or P815 cells. Mice were injected i.p. with LFn-NP_118–404 plus PA or LFn-NP_118–404 alone. After in vitro stimulation, samples were assayed for their ability to lyse ⁵¹Cr-labeled EL-4 or P815 cells coated with the cognate peptide (closed circles) or not coated (open circles). The cultures tested in this assay were splenocytes from individual mice immunized with LFn-NP_118–404 plus PA (A) or LFn-NP_118–404 in the absence of PA (B). Targeting was evaluated by measuring ⁵¹Cr release. Threefold effector dilutions are shown. Similar levels of lysis were observed for each of three replicates.