Fig. 2.

Schematic overview of ZFP57 isoforms and regions used for analysis in this study. Top, overview of human chromosome 6 (genome release hg19), with ZFP57 alternative isoforms (dark blue) starting at a differentially methylated region (green) and a CTCF binding site (grey) with insulator activity (light blue). The mQTLs of interest is highlighted in black. Bottom, a zoomed-in section of the differentially methylated region (green) within the first ZFP57 intron with primers used for DNA methylation (CpG pyroassay) and genotype (SNP pyroassay) analysis. Informative SNP variants from this study are shown, the mQTLs are shown in black and some of the other SNPs in the region also shown. The CTCF binding site (grey) and insulator (light blue) are shown in comparison to primer and SNP locations