Table 2.

Epigenetic characteristics in cord blood and blood from children at age 11

		Placebo			Folic acid				p value
Characteristics	No. CpG	N	Mean	SD	N	Mean	SD	Change in methylation (%)
ZFP57 DMR methylation (Cord blood)	6	52	61.22	18.51	41	66.36	16.78	5.14	0.169
ZFP57 DMR methylation (11-year blood)	6	13	54.53	9.48	10	56.57	8.64	2.04	0.601
ZFP57 DMR methylation (EPIC 850 k array)	15	45	64.38	14.63	41	70.61	12.22	6.23	0.012

Statistical comparisons by independent t-test (cord blood and 11-year blood) and using Limma to generate comb-p value for EPIC array. DMR methylation performed by pyrosequencing of 6 CpG sites (cord blood and 11-year blood) and by EPIC array

SD standard deviation, N number of samples

^*p < 0.05