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. 2024 Oct 28;61(6):304–317. doi: 10.1159/000541651

Fig. 3.

Fig. 3.

In vivo administration of Pam3CSK4 attenuates AngII-induced effects of macrophage infiltration and M1/M2 polarization. a Groups of mice were treated with AngII, a combination of AngII and Pam3CSK4 (Pam3+AngII) or placebo (control). Flow cytometric analysis demonstrated a significant increase in total macrophage (CD11b+Lin−F4/80+) infiltration in AngII-treated mice relative to controls. The treatment of mice with Pam3CSK4 significantly decreased AngII-induced total macrophage infiltration in the abdominal aortas (n = 5/group). b, c CD11b+Lin−F4/80+ cells were analyzed for the expression of CD11c (M1) or CD206 (M2). The ratio of expression of M1-associated markers relative to M2-associated markers was significantly increased by AngII treatment. Pam3CSK4 treatment markedly decreased the M1/M2 ratio at (panel b) day 10 (n = 6/group) and (panel c) day 28 (n = 5/group). **p < 0.01 versus control, ##p < 0.01 versus AngII alone.