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. 1999 Jul;67(7):3317–3328. doi: 10.1128/iai.67.7.3317-3328.1999

FIG. 2.

FIG. 2

Identification of H. ducreyi isolates, detection of homology to hemolysin genes, and phenotypic expression of hemolysin in geographically and temporally diverse isolates of H. ducreyi. (A to C) Spot blots of H. ducreyi and other Haemophilus species probed with whole-cell DNA from H. ducreyi type strain CIP 542 (A), the hhdB gene (B), and the hhdA gene (C). Strains and species analyzed: top row, CIP542, 35000, LA228R, CF101, V-1168, V149/91, V-180, HMC56, CHIA, and V-1157; bottom row, H. influenzae Rd, H. parainfluenzae, H. aphrophilus, H. paraphrophilus, H. segmis, A. pleuropneumoniae, H. haemoglobinophiles, T. equigenitalis, H. haemolyticus, and H. influenzae ATCC 33911. (D) Hemolysis of H. ducreyi isolates on HBAPs. Isolates on HBAPs correspond to those in the top rows of panels A to C except strain 35000-KmA, the right-most strain in panel D, which was added as a nonhemolytic control. The photographs of the Southern blots and the HBAP were scanned with a Microtek Scanner III and captured with a Micrografx Picture Publisher (Micrografx, Richardson, Tex.).