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Production of antibodies to H. ducreyi hemolysin by patients diagnosed with chancroid. Each lane contains 200 ng of purified His-tagged hemolysin separated on an 8% polyacrylamide gel, blotted to nitrocellulose, and probed with human serum from five different chancroid-positive patients (lanes 1 to 5) and from five different chancroid-negative controls (lane 6 to 9). The blots were scanned with a Microtek scanner, and intensities of the bands are shown at the bottom.
