TABLE 2.

Bacterial strains and plasmids used in this study

Strain or plasmid	Description	Source or referencea
Strains
A. pleuropneumoniae	ATCC 27088	Arnold Smith
E. coli DH5α	Host for cloning experiments	GibcoBRL, Gaithersburg, Md.
E. coli BL21(DE3)	Host for protein expression	Novagen, Madison, Wis.
H. aphrophilus	ATCC 33389	Arnold Smith
H. ducreyi 35000-TcA	Strain 35000 with Tn916 inserted in hhdB, nonhemolytic	50
H. ducreyi 35000-KmA	Strain 35000 with Tn1545-Δ3 inserted in hhdB, nonhemolytic	50
H. ducreyi 35000ΔAPC	Strain 35000 with cat cassette in hhdA gene, nonhemolytic	54
H. haemoglobinophilus	ATCC 19416	ATCC, Manassas, Va.
H. haemolyticus	ATCC 33390	Arnold Smith
H. influenzae	ATCC 33911	Arnold Smith
H. influenzae Rd		Marilyn Roberts
H. parainfluenzae	ATCC 33392	Arnold Smith
H. paraphrophilus	ATCC 29237	Arnold Smith
H. segmis	ATCC 33393	Arnold Smith
T. equigenitalis	ATCC 35865	Arnold Smith
Plasmids
pUC19	E. coli cloning vector, Apr	GibcoBRL
pCR2.1	TA cloning vector for cloning PCR products, Ampr Kanr	Invitrogen, San Diego, Calif.
pET24+	Expression plasmid with C-terminal His tag sequence and inducible T7 promoter, no translation initiation signals, Kanr	Novagen
pET24a+	Expression plasmid with C-terminal His tag sequence and inducible T7 promoter, Kanr	Novagen
pBCKS	Cmr plasmid derived from pUC19 with KS multiple cloning site	Stratagene, La Jolla, Calif.
pPT384-ETa	hhdBA genes cloned into pET24a+ SstI-SalI	This study
pLSSK	Shuttle vector with ori, sulA, and strA genes from pLS88 and multiple cloning sites and lacZ gene from pBluescript SK	54
pPT384	hhdBA gene region in pTZ18 in same orientation as lac promoter	50
pPT376BCKS	5.8-kb BglII fragment containing part of hhdB and all of hhdA cloned into pBCKS SstI-SalI	50
pETBABN	hhdBA genes cloned into pET24+ BamHI-SalI	This study
pLSBAHis+	hhdBA genes cloned into pLSSK in same orientation as lac promoter. His tag sequence from pET24+ is fused to 3′ end of hhdA	This study

^aAffiliations: Arnold Smith, University of Missouri, Columbia; Marilyn Roberts, University of Washington.