TABLE 3.
Oligonucleotide primers used in this study
| Name | Target | Positiona | Strand | Sequence 5′-3′b | Comment |
|---|---|---|---|---|---|
| hhdBstart | hhdBA | 209–237 (U32175) | + | gga tccaag gag GAT AAT ATG AGA AGA TGT GAA ATT ATA AC | BamHI site and RBS added |
| revAend | hhdBA | 5421–5398 (U32175) | − | gtc gac TCG AAT GGC CAT CTT AGC ATC GAC | SalI added |
| hhdAstart | hhdA | 1889–1909 (U32175) | + | gga tccaag gag ATA CAT ATA TGA AAA AAT GGA AGC C | BamHI site and RBS added |
| BglR | hhdBA | 785–764 (U32175) | − | CCA AAT CTA AAT GAA TAC CGC C | |
| 1545-1 | Tn1545-Δ3 | Left end of Tn1545-Δ3 (5) | − | GAT AAA GTG TGA TAA GTC CAG | |
| 1545-2 | Tn1545-Δ3 | Right end of Tn1545-Δ3 (5) | − | CAC ATA GAA TAA GGC TTT ACG | |
| Tn916 | Tn916 | Right end of Tn916 (9) | − | GAG TGG TTT TGA CCT TGA |
a
Position on target sequences. Numbers are based on given reference or GenBank citation.
b
Homologous sequences are in uppercase, restriction sites are underlined, and ribosome binding sites (RBS) are double underlined.