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Full mass spectra of Pyruvate kinase (5 μM) in 200 mM ammonium acetate with increasing amounts of in-source activation (isCID) to remove non-specific adducts. As adducts were lost, the apex of each charge state decreased as demonstrated by the red line placed at the apex of 38+ for no activation (A-C). At high isCID, GBP adductions began to be resolved.
