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(A) Indel mutations revealed by DNA sequencing of STAT3 alleles in STAT3-KO cells generated with the CRISPR/Cas9 system. Mutations are indicated in red. (B) Western blot analysis confirmed the loss of Stat3 gene expression. (Lane 1: protein ladder; Lane 2: wild type control BMSC; Lane 2: STAT3-KO BMSC).
