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Alcian blue was used to stain sulphated glycosaminoglycans in cartilage and alizarin red for hydroxyapatite in bones. Representative male skeletons are displayed for wild type (WT) control and STAT3-OKO-M pups (Top two panesl). Zoomed views of the control (1, 3, 5 and 7) and STAT3-OKO-M (2, 4, 6, and 8) skeletons, showing patellar and tarsal joints (1 and 2) calvaria (3, 4), lumbar vertebrae (5 and 6), and elbow and carpal joints (7 and 8).
