Table 1. Functional connections between Hsc82 mutants, cochaperones and clients.

A comparison between data in this paper regarding the client eEF2 with prior data. Results from the current study are shaded in gray. (*nd = not determined).

Client function analyzed	eEF2 folding	eEF2 sensitivity to DT	Utp21	Cyr1	v-src kinase
Cochaperone requirement	HGH1 required to maintain steady state levels of eEF2	Deletion of STI1 results in enhanced resistance to DT	Deletion of STI1 results in severe growth defect of cells expressing utp21 mutation	Mutation in SGT1 affects cAMP pathway	CDC37 required for v-src activity
Hsp90 mutant effect on client	Reopening mutants have biggest effect on steady state levels of eEF2	Loading and closing mutants have enhanced resistance to DT	Loading and closing mutants exhibit severe growth defect when combined with utp21 mutation.	W296A mutation affects cAMP pathway	Loading and closing mutants have biggest effect on v-src activity
Cochaperone-Hsp90 connection	Reopening mutants have severe growth defect in absence of HGH1	Loading and closing mutants have severe growth defect in absence of STI1	Loading and closing mutants have severe growth defect in absence of STI1	n.d.	Loading and closing mutants have severe growth defect when combined with cdc37 mutation
References	[34], This study	This study, [35]	[35,74,79]	[39]	[35]