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. 2024 Dec 17;7:0551. doi: 10.34133/research.0551

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Copyright © 2024 Jiashu Yang et al.

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Fig. 4. — HSP90α stabilized the formation of NLRP3 complexes in 3 stages. (A) An endogenous IP with HSP90α antibody was performed in LPS and/or ATP-stimulated BMDMs. (B) HEK293T cells were transfected with HA-NEK7, flag-NLRP3, or GFP-ASC alone or cotransfected with HA-NEK7 and flag-NLRP3, or HA-NEK7, flag-NLRP3, and GFP-ASC. IP and Western blotting analysis of the interaction between HSP90α and 3 stages of NLRP3 complexes. (C) GFP-Vector, (D) Cage-NLRP3, (E) NLRP3-NEK7 complex, and (F) NLRP3-NEK7-ASC complex were reproduced in HEK293T cells. Colocalization of HSP90α and 3 stages of NLRP3 complexes was determined by IF. Scale bar, 10 μm.