FIG. 2.

(A) PCR analysis of wild-type and hemolysin BL-deficient strains. Samples of chromosomal DNA from strains MGBC145 (lane 1) and CJ145-1.1 (lane 2) were used to amplify hemolysin BL. PCR products were of the predicted sizes (indicated by lines on the left). (B) Chromosomal DNA from strains MGBC145 (lanes 1 and 3) and CJ145-1.1 (lanes 2 and 4) were digested with EcoRI, and fragments were separated on a 0.8% agarose gel. Lanes 1 and 2 were probed with ³³P-labeled pKRX-hbl. Lanes 3 and 4 were probed with ³³P-labeled pUC18-ermAM. MW, molecular weight markers.