| Antibodies |
| Rabbit polyclonal anti-TEX14, 1:200 |
Abcam |
Cat# ab41733 |
| Rabbit polyclonal anti-DDX4, 1:400 |
Abcam |
Cat# ab13840 |
| Mouse monoclonal AF 647 anti-SYCP3, 1:100 |
Abcam |
Cat# ab205847 |
| Mouse monoclonal AF 647 anti-MgcRacGAP, 1:100 |
Santa Cruz Biotechnology |
Cat# sc-271110 |
| Chicken polyclonal anti-GFP, 1:400 |
Abcam |
Cat# ab13970 |
| Donkey AF 488 anti-rabbit, 1:500 |
Invitrogen |
Cat# A21206 |
| Donkey AF 488 anti-chicken, 1:500 |
Abcam |
Cat# ab13970 |
| Donkey AF 647 anti-rabbit, 1:500 |
Invitrogen |
Cat# A31573
|
| Hoechst 3342 solution, 20 mM, 1:10,000 |
Thermo Fisher |
Cat # 62249 |
| Chemicals, peptides, and recombinant proteins |
| Tamoxifen |
Sigma |
Cat# T5648 |
| Kolliphor EL |
Sigma |
Cat# C5135 |
| Matrigel Basement Membrane Matrix |
VWR |
Cat# 47743-722 |
| DMEM/F-12 HEPES, no phenol red |
Fisher/Gibco |
Cat# 11039021 |
| Fetal bovine serum |
Bio-techne |
Cat# S11550 |
| CK-666 |
Millipore-Sigma |
Cat# SML0006-5MG |
| DMSO |
Sigma |
Cat# D2650-5X5ML |
| Experimental models: Organisms/strains |
| Mouse: mTmG: (B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
|
Gift from Dr. Danelle Devenport |
RRID:IMSR_JAX:007676 |
| Mouse: Oct4Cre-MerCreMer: B6(SJL)-Pou5f1tm1.1(cre/Esr1*)Yseg/J
|
The Jackson Laboratory |
RRID:IMSR_JAX:016829 |
| Mouse: H2B-miRFP740
|
Nunley et al51
|
N/A |
| Mouse: CD-1 IGS |
Charles River Laboratory |
Strain #022 |
| Oligonucleotides |
| Primer: Tex14 knockout forward: GGCTAACTGGTGTGAGTGGA |
This paper |
N/A |
| Primer: Tex14 knockout reverse: TTCCTGACTCCAAGCCTAGC |
This paper |
N/A |
| Primer: Rbm31 forward: CACCTTAAGAACAAGCCAATACA |
Tunster et al52
|
N/A |
| Primer: Rbm31 reverse: GGCTTGTCCTGAAAACATTTGG |
Tunster et al52
|
N/A |
| Recombinant DNA |
| sgRNA: Tex14 knockout: AGGCUUUGGGAAUGAAAAGG |
This paper |
N/A |
| sgRNA: Tex14 knockout: GCUGUAAUGAAGGUCCCCCA |
This paper |
N/A |
| Software and algorithms |
| FIJI |
https://imagej.net/software/fiji/downloads
|
RRID:SCR_002285 |
| Napari |
https://zenodo.org/records/13863809
|
RRID:SCR_022765 |
| Cellpose 2.0 |
Stringer et al.54
|
RRID:SCR_021716 |
| Scikit-image |
https://github.com/scikit-image/scikit-image
|
RRID:SCR_021142 |
| NIS Elements Analysis |
Nikon |
RRID:SCR_014329 |
| SnapGene |
Dotmatics |
RRID:SCR_015052 |
| MATLAB 2022a |
MathWorks |
RRID:SCR_001622 |
| Excel 2019 |
Microsoft |
RRID:SCR_016137 |
| Probabilistic fracture model |
This paper |
https://zenodo.org/records/11263587
|
