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. 2024 Dec 17;13(6):e70013. doi: 10.1002/mbo3.70013

Figure 1.

Figure 1

Schematic representation of the procedure used in the experimentation. Chlamydomonas and Scenedesmus were inoculated at 2 × 10⁵ cells/mL in 200 mL TAP media and grown under light with continuous shaking for 3 days until they reached mid‐log phase. Asynchronized cells were diluted to 4.5–4.7 × 10⁶ cells/mL and divided into four groups: two for liquid cultures and two for immobilization. For immobilization, pellets were resuspended in 2% Na‐alginate, and droplets were polymerized in 2% CaCl₂ to form ~4 mm beads (20 beads/culture). Beads were washed and introduced into DCF media. TAP media served as negative control, while TAP + 150 µM DCF without algae or with empty beads served as positive controls. “Created with BioRender.com.”