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. 2024 Dec 7;16(12):evae267. doi: 10.1093/gbe/evae267

Fig. 5.

Fig. 5.

Evolution pattern and expression of AP2/ERF-like TFs. a) Phylogenetic analysis of legume AP2/ERF-like TFs. Members of DEGs are labeled on the tree using different shapes. b) Analysis of microsynteny and gene duplication events of candidate AP2/ERF-like TFs among the indicated four genera of legume species. DSD, dispersed duplication; TRD, transposed duplication; TD, tandem duplication; WGD, whole-gene duplication. c) Tissue expression patterns of candidate AP2/ERF-like TFs in soybean. Expression data are from ePlants (https://bar.utoronto.ca). In b) and c), highlighted and bold genes are DEGs. d to f) Relative expression level of Glyma.17G145300.1 d), Glyma.05G063600.1 e), and Glyma.20G195900.1 f) in soybean pods at early and later stages. Total RNA from cultivar SN14 and wild soybean ZYD06 was subjected to qRT-PCR. In d) to f), different letters (a to c) indicate significant difference (P < 0.05) in one-way ANOVA. Each bar corresponds to the mean value ± SD of three biological replicates.