FIG. 7.
Effect of neuraminidase on WGA-induced NADPH-oxidase activation. Responding (fMLP 10′) neutrophils were treated with 0.2 U of neuraminidase per ml for 5 min on ice and washed twice. The results are expressed as the peak values of the CL responses induced by WGA (2 μg/ml) in control (open bars) or neuraminidase-treated (closed bars) neutrophils (105 cells). The extracellular responses were measured in the presence of isoluminol (5 × 10−5 M) and HRP (4 U), while the intracellular responses were measured in the presence of luminol (5 × 10−5 M), SOD (50 U), and catalase (2,000 U). Shown are the means plus standard deviations (error bars) of four experiments. The CL is given in increments of 106 cpm (Mcpm).