Abstract
1. The range of fatty acids formed by preparations of ultrasonically ruptured avocado mesocarp plastids was dependent on the substrate. Whereas [1-14C]palmitate and [14C]oleate were the major products obtained from [-14C]acetate and [1-14C]acetyl-CoA, the principal product from [2-14C]malonyl-CoA was [14-C]stearate. 2. Ultracentrifugation of the ruptured plastids at 105000g gave a supernatant that formed mainly stearate from [2-14C]malonyl-CoA and to a lesser extent from [1-14C]acetate. The incorporation of [1-14C]acetate into stearate by this fraction was inhibited by avidin. 3. The 105000g precipitate of the disrupted plastids incorporated [1-14C]acetate into a mixture of fatty acids that contained largely [14C]plamitate and [14C]oleate. The formation of [14C]palmitate and [14C]oleate by disrupted plastids was unaffected by avidin. 4. The soluble fatty acid synthetase was precipitated from the 105000g supernatant in the 35-65%-saturated-(NH4)2SO4 fraction and showed an absolute requirement for acyl-carrier protein. 5. Both fractions synthesized fatty acids de novo.
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- BARRON E. J., SQUIRES C., STUMPF P. K. Fat metabolism in higher plants. XV. Enzymic synthesis of fatty acids by an extract of avocado mesocarp. J Biol Chem. 1961 Oct;236:2610–2614. [PubMed] [Google Scholar]
- Brooks J. L., Stumpf P. K. Fat metabolism in higher plants. XXXIX. Properties of a soluble fatty acid synthesizing system from lettuce chloroplasts. Arch Biochem Biophys. 1966 Sep 26;116(1):108–116. doi: 10.1016/0003-9861(66)90019-1. [DOI] [PubMed] [Google Scholar]
- Delo J., Ernst-Fonberg M. L., Bloch K. Fatty acid synthetases from Euglena gracilis. Arch Biochem Biophys. 1971 Apr;143(2):384–391. doi: 10.1016/0003-9861(71)90225-6. [DOI] [PubMed] [Google Scholar]
- Ernst-Fonberg M. L., Bloch K. A chloroplast-associated fatty acid synthetase system in Euglena. Arch Biochem Biophys. 1971 Apr;143(2):392–400. doi: 10.1016/0003-9861(71)90226-8. [DOI] [PubMed] [Google Scholar]
- Kannangara C. G., Jacobson B. S., Stumpf P. K. Fat Metabolism in Higher Plants: LVII. A Comparison of Fatty Acid-Synthesizing Enzymes in Chloroplasts Isolated from Mature and Immature Leaves of Spinach. Plant Physiol. 1973 Aug;52(2):156–161. doi: 10.1104/pp.52.2.156. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nagai J., Bloch K. Synthesis of oleic acid by Euglena gracilis. J Biol Chem. 1965 Sep;240(9):3702–3703. [PubMed] [Google Scholar]
- Simoni R. D., Criddle R. S., Stumpf P. K. Fat metabolism in higher plants. XXXI. Purification and properties of plant and bacterial acyl carrier proteins. J Biol Chem. 1967 Feb 25;242(4):573–581. [PubMed] [Google Scholar]
- Stumpf P. K., Boardman N. K. Fat metabolism in higher plants. XXXIX. Effect of adenosine triphosphate and triton X-100 on lipid synthesis by isolated spinach chloroplasts. J Biol Chem. 1970 May 25;245(10):2579–2587. [PubMed] [Google Scholar]
- Weaire P. J., Kekwick R. G. The synthesis of fatty acids in avocado mesocarp and cauliflower bud tissue. Biochem J. 1975 Feb;146(2):425–437. doi: 10.1042/bj1460425. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YANG S. F., STUMPF P. K. FAT METABOLISM IN HIGHER PLANTS. XXI. BIOSYNTHESIS OF FATTY ACIDS BY AVOCADO MESOCARP ENZYME SYSTEMS. Biochim Biophys Acta. 1965 Feb 1;98:19–26. [PubMed] [Google Scholar]