TABLE 1.
Adherence to and invasion of HEC-1-B cells by P+ Opa+ variants of strains MS11mkC and FA1090 OpaE after 4 h of incubation
| Inoculum | MS11mkC
|
FA1090 OpaE
|
||||
|---|---|---|---|---|---|---|
| Adherence (CFU, 104)a | Invasion
|
Adherence (CFU, 104) | Invasion
|
|||
| CFU (102)b | %c | CFU (102) | % | |||
| 1 × 107–2 × 107 | 3.6 | 2.2 | 0.6 | 1–2 | 2–3.7 | 2.0 |
| 1 × 106–2 × 106 | 3.6 | 0.6 | 0.2 | 1–2 | 0.5–1 | 0.5 |
| 1 × 105–2 × 105 | 1.6 | 0.4 | 0.25 | 0.5–1 | 0.1–0.4 | 0.3 |
| 1 × 104–2 × 104 | 0.3 | 0.1 | 0.33 | 0.1–0.2 | 0.1–0.2 | 1.0 |
a
CFU recovered after 4 h of incubation in the absence of gentamicin (total CFU). Because of microcolony formation, adherence is variously proportional to, but not the same as, the proportion of the inoculum that initially adhered to the HEC-1-B cells.
b
CFU recovered after 4 h of incubation in the presence of gentamicin (intracellular CFU).
c
The proportion of total CFU that were intracellular. Because of microcolony formation, this value is not the proportion of the inoculum that invaded.