Figure 6.

TβRIII is involved in a detrimental action of TGFβ2 on SGOs. a) Representative WB image detecting TβRIII in the SG of cont‐ and OVX mice (n = 5 for each). b,c) The impact of TGFβ2 on TβRIII expression in SGOs was investigated with flow cytometry (b) and WB (c). d–f) Representative images of shCont‐ and shTβRIII‐SGOs after TGFβ2 exposure (d) and their growth parameters (e) as well as viability assessment (f). g) Lipid peroxidation levels in shCont‐ and shTβRIII‐SGOs were evaluated after TGFβ2 treatment. TGFβ2 was administered on culture day 5 for 72 h (c‐e) or 24 h (f, g). At least three lines of SGOs were used for all experiments. Hoechst staining was conducted in (g) to visualize each organoid for the analysis. Scale bar = 300 µm (d, f) and 1 mm (g). Data are shown as the mean ± SEM and compared by unpaired t‐test (b, c, g) or one‐way ANOVA with Dunnett`s multiple comparisons tests (e, f). *P < 0.05, **P < 0.01, ***P < 0.001.