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. 2024 Nov 1;11(47):2400660. doi: 10.1002/advs.202400660

Figure 7.

Figure 7

In vivo administration of ferroptosis inhibitor contributes to SG recovery in OVX mice. a) Experimental timeline schematic for the animal study. b) Immunoblot analysis showing the influence of Lip‐1 treatment on the regulation of ferroptosis, lipid peroxidation and TGFβ2 level in the OVX‐SGs. c,d) Representative images of MDA immunostaining (c) and quantification of MDA levels (d) within the SG tissues, demonstrating the beneficial impact of Lip‐1 treatment in reducing lipid peroxidation levels in OVX‐SGs. e) The quantity of acini in the SG was estimated by AQP5 immunostaining. f) SG images labeled with CK14 and assessment of the proportion of CK14+ ducts. CK14‐expressing acini and duct are indicated by yellow and white arrowheads, respectively. g) 7 weeks after OVX or sham surgery, the saliva secretory capacity of each group upon pilocarpine injection was measured. A total 4 mice for each group were used for histological analysis. The number of biological replicates for the WB analysis, MDA measurement and saliva measurement corresponds to the number of dots on the graph. In (c, e, f), F‐actin staining was performed to indicate the epithelial structure of the SG. Scale bar = 40 µm. Data are shown as the mean ± SEM and compared by unpaired t‐test (e, f) or one‐way ANOVA with Dunnett's multiple comparisons tests (d, g). *P < 0.05, **P < 0.01. For (g), # P < 0.05, where the statistical significance was determined by unpaired t‐test.