Figure 5.

Spatiotemporal analysis of dental papilla development with spatial transcriptome sequencing and scRNA‐seq. a) The developmental connections of dental papilla domains. b) UMAP plot visualizing dental papilla cell clusters (i) and papilla cell distribution based on the developmental time course (ii) and cell cycle stage (iii). c) Pseudotemporal cell ordering visualizes the developmental course of dental papilla clusters. d) Conjoint analysis of single cell and spatial transcriptome sequencing representing the spatial distribution characteristics of the dental papilla clusters. e) Dot plot representing markers of specific cell clusters in the dental papilla compartment. Points are scaled by the percentage of cells expressing the gene within the cluster and colored by the average expression level. f) Bar plots representing the time distribution characteristics of dental papilla clusters, indicating the occurrence sequence of progenitor clusters and differentiated clusters. g) A T‐SNE plot showed the marker genes of five dental papilla clusters. h) Conjoint analysis of single cell and spatial transcriptome sequencing showing the spatial distributions of cusp progenitor DPs, cusp early DPs, and Odontoblast at the bud stage, cap stage, bell stage, and the differentiation stage.