FIG. 2.

Total numbers of IEL (a) and ratios of the number of αβIEL to that of γδIEL (b) in the small intestines of GF mice, SFB-mice, Clost-mice, mice associated with both SFB and clostridia (SFB + Clost), and Cvd mice. The gnotobiotic mice were analyzed at 16 weeks of age in the second generation and compared with age-matched GF mice. The data represent means plus the standard deviations (n = 3 or 4) and were also reproducible in the experiment involving the first generation. The letters a, b, c, and d near the columns indicate statistically significant differences from the SFB, Clost, SFB + Clost, and Cvd groups, respectively. Single letter, P < 0.05; double letters, P < 0.01.