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L. monocytogenes from infected PBL can spread to endothelial cells. PBL were isolated from L. monocytogenes-infected mice. They were cocultured with (□, ■) and without (○, ●) monolayers of TNF-α-stimulated endothelial cells for 1 and 18 h in the presence of 10 μg of gentamicin per ml. Results presented are the mean log10 CFU of bacteria/well (±SEM) from triplicate wells from two identical experiments (open and filled symbols indicate the separate results of the two experiments).
