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. 2005 Jul;16(7):3107–3116. doi: 10.1091/mbc.E05-02-0103

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Copyright © 2005, The American Society for Cell Biology

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Figure 7. — Dynamin localization at the investment cones is reduced in ddlc1 testes. (A and B) Anti-dynamin (green) and RITC:phalloidin (red) staining of the wild-type ICs. Dynamin is found along the cone (arrow) excluding the leading edges (arrowheads). (C) Dynamin (green) is colocalized with DDLC1 (magenta) at the F-actin cones (arrows). (D) Dynamin (green) and RITC:phalloidin (red) staining at the cones (arrows) after only buffer (control), colchicine and latrunculin B treatments. (E and F) Gray levels of RITC:phalloidin and dynamin stainings at the ICs in specimens from ddlc1^ins1 testes before (E) and after an 11-h (F) heat pulse are displayed in a false color scale (as depicted in F). (F) Dynamin staining is considerably reduced at the cones (arrows) after the heat pulse. (G) A typical IC from a shi^ts2 hemizygous testis after a 16-h pulse shows loss of F-actin staining from the cones and diffused DDLC1 staining in the cytoplasm. Dynamin staining remained along the cones (arrow), but it was less compact than those found in the control (shi^ts2 without heat pulse). Scale bar in A, 10 μm.