Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Dec 18;55:169. doi: 10.1186/s13567-024-01415-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Screening of immunodominant epitopes of H5N1 AIV-specific CD8⁺T cells. A RT-PCR analysis of IFN-γ gene expression in B1 haplotype memory PBMCs stimulated with peptide pools. The data were collected from three replicates of three biological samples. B RT-PCR analysis of IFN-γ gene expression in B1 haplotype memory PBMCs with individual 9-mer peptide stimulation from positive peptide pools. The results are presented as the means ± SEM, and paired t tests were used for statistical comparisons. *P < 0.05, **P < 0.01, ***P < 0.001. C Conservation of the sequences between circulating strains for the positive peptides. The Global Initiative of Sharing All Influenza Data (GISAID) (gisaid.org) was used with the search criteria set as Asia, 2019 to 2024, NA/NP/M/PB1/PA/NS1, and H5N1/H5N6/H5N8/H7N9/H9N2. Protein sequences were aligned with the MUSCLE algorithm. The frequency of mutation was determined. The protein sequences of influenza A (H5N1-H5N6-H5N8-H7N9-H9N2) viruses are represented by various colored bars, and the number above the bars indicates the number of virus strains.