FIG. 1.

ORF map of a 24,454-bp region of the 9343 chromosome contained on cosmid pMJC2. Restriction sites within this region are shown for the following enzymes: PvuII (P), EcoRI (E), Asp718 (A), StuI (St), SmaI (Sm), SalI (Sl), MluI (M), and Sau3AI/BamHI junction with the pHC79 vector (Sa/B). ΔwcfD-L and ΔwcfF represent the regions that were deleted in the chromosomes of mutants 9343ΔwcfD-L and 9343ΔwcfF. The arrows indicate direction of transcription of each ORF. The ORFs that do not demonstrate homology with genes involved in polysaccharide biosynthesis (outside the wcf locus) are shaded. The lower diagram demonstrates the average G+C content for each ORF of the region. The dashed-line boxes represent a clustering of adjacent ORFs with similar G+C contents. A transcriptional terminator-like stem-loop region downstream of wcfL is indicated.