Figure 6.

Defects in spindle organization in Asp-depleted S2 cells. Asp RNAi-treated cells displayed longer spindles than controls with detached centrosomes (D, E, F, red arrows) and unfocused, disorganized spindle poles. In some cells, centrosomes remain loosely attached to the spindle (A, B, and C; white arrows) with apparent fragmentation of γ-tubulin foci (C; white arrow). Chromosomes are frequently misaligned (C and E). Immunofluorescent localization of α-tubulin (green), γ-tubulin (red), and DNA (blue). Bar, 10 μm.