Figure 2.

GRASP65 depletion does not affect the organization of Golgi membranes. (A) HeLa cells were transfected with GR65–1–scrambled (scrambled siRNA) and GR65–1 (GRASP65-specific siRNA) and plated on coverslips. Forty-eight to 60 h after transfection, cells were fixed and processed for immunofluorescence microscopy using the indicated antibodies. To detect mannosidase II, a HeLa cell line that stably expresses mannosidase II-GFP was used for transfection. (B) HeLa cells untreated (a, control), transfected with GR65–1 (b), or GR65–1–scrambled (c) were cultured for 48 h. The cells were processed for electron microscopy as described in Materials and Methods. The number of Golgi cisternae per stack was quantified from 30 cells per condition (d).