Figure 3.

Actin protein alignment and sequence changes caused by act-5 mutations. C. elegans actin protein alignment. ClustalW 1.83 alignment (Thompson et al., 1994) between the five C. elegans actins (CeACT-1–5, accession nos. CAB04678, CAB04675, CAB04676, AAB04575, and CAB05817, respectively) and the predicted Caenorhabditis briggsae ACT-5 (CbACT-5, accession no. CAE71353). The protein sequence for C. elegans ACT-1 and ACT-3 are identical and denoted as CeACT-1_3. Identical residues are shaded in black, conserved changes in gray. Sequence coordinates are given for the entire alignment, which is offset 1 residue for CeACT-5 and CbACT-5. The boxed region at residues 220–232 indicates the synthetic peptide used to elicit anti-ACT-5 antibodies. Asterisk indicates the position of the premature stop codon in dt2019; down arrows denote the region deleted in dt2017 and replaced by asparagine.