Figure 5.

Intestinal cell polarization does not require act-5 function. MH27 staining (white) labels apical regions of polarized cells, giving rise to a web-like pattern along the bodies of the wild-type (left) and act-5(dt2017) mutant (right) worms. Although act-5 mutant intestinal lumens seem slightly deformed relative to control animals, MH27 is properly restricted to apical cell regions in both genotypes. Projections encompass the entire volume of each animal. Anterior (ant.) and posterior (post.) ends of the worm are indicated. Bar, 10 μm.