Figure 5.

Elongation of wild-type and Glo mutant embryos. Images of wild-type (A–C) and glo-1 (D–F) embryos were captured at the times listed after first cell cleavage. glo-1 embryos elongated normally and reached the fourfold stage (E and G), however soon after they reduced to 3-fold in length (F and G) and displayed ripples in their epidermis/cuticle (F, black arrows). glo-2 (H) and glo-3 (I) embryos similarly elongated to fourfold before reducing to 3-fold length. (G–I) Glo embryos lacked birefringent gut granules before they occurred in the intestinal lumen (arrow denotes average time of occurrence). In G–I, the length of individual embryos and the presence/localization of birefringent material was monitored every 45 min starting at the 1.5-fold stage. Bars represent 95% confidence interval.