Figure 10.

TAT-ELKSBsnBD binds Bassoon (A) and inhibits the binding of ELKS and Bassoon. (A) Direct binding of TAT-ELKSBsnBD to Bassoon by pull-down assay. The GST fusion proteins containing Bassoon and Piccolo as well as GST alone were immobilized to glutathione-Sepharose beads. Myc-tagged TAT-ELKSBsnBD was then incubated with the beads. Proteins that bound to the beads were analyzed by immunoblotting by using anti-Myc mAb. Arrowheads indicate TAT-ELKSBsnBD. Note that TAT-ELKSBsnBD found GST-Bassoon and GST-Piccolo, but it did not bind GST alone. (B) Effects of TAT-ELKSBsnBD on the binding of ELKS and Bassoon. Immunoprecipitation assay of Myc-ELKS and EGFP-Bassoon was performed in the presence or absence of Myc-tagged TAT-ELKSBsnBD by using anti-GFP antibody, followed by immunoblotting by using the anti-Myc and anti-GFP antibodies. Note that the binding of ELKS and Bassoon was inhibited in the presence of TAT-ELKSBsnBD.