Figure 12.
Effects of TAT-ELKSBsnBD on insulin release and on [Ca2+]i response to high glucose in MIN6 cells. (A) Insulin release from perifused control and the TAT fusion protein-treated (70 μg/ml for 50 min) cells stimulated with 22 mM glucose. The cells in the cell chamber (∼106 cells per chamber) were perifused with KRB (0.5 ml/min) at 37°C, and the perfusate was analyzed for IRI by radioimmunoassay. Results are given as a percentage of the cellular insulin content (2.9 μg/106 cells in control cells, 3.1 μg/106 cells in TAT-ELKSContD-treated cells, and 2.8 μg/106 cells in TAT-ELKSBsnBD–treated cells). (B) Glucose-induced changes in [Ca2+]i in control and the TAT fusion protein-treated (70 μg/ml for 50 min) cells. Glucose-induced changes in [Ca2+]i were measured in each treated cells by Fura-2 AM (5 μM). Time 0 indicates the time of the addition of high glucose. The fluorescence ratio (340/360) at time 0 was taken as 1.