Table 1.

FRET_R values of pairwise combinations of SPB core proteins

CFP donor	YFP acceptor	FRETR	SD	N	Strain
C:Cmd1	N:Spc110	1.01	0.11	95	BESY102-2A
C:Cmd1	C:Spc42	1.06	0.07	99	BESY97-3D
C:Cmd1	C:Cnm67	1.09	0.1	102	BESY98-2D
C:Cmd1	C:Spc29	1.68	0.16	84	BESY100-3B
C:Cmd1	N:Spc29	1.75	0.23	93	BESY101-4C
C:Cmd1	C:Spc110	1.94	0.21	93	BESY99-6C
C:Cmd1	N:Spc42	2.04	0.21	90	BESY96-1D
C:Cnm67	N:Spc110	0.99	0.07	77	EMY178
C:Cnm67	N:Spc29	0.99	0.1	69	EMY167-1D
C:Cnm67	C:Spc110	1.02	0.06	79	EMY175
C:Cnm67	C:Cmd1	1.1	0.08	102	BESY95-6D
C:Cnm67	C:Spc29	1.1	0.11	70	EMY192
C:Cnm67	N:Spc42	1.15	0.08	77	EMY176
C:Cnm67	C:Spc42	2.52	0.19	81	EMY164-1D
Cnm67-YFP-CFP	Cnm67-YFP-CFP	2.65	0.18	64	DHY151
YFP-Spc110-CFP	YFP-Spc110-CFP	1.02	0.06	132	EMY173
C:Spc110	C:Cnm67	1.05	0.06	79	DHY71
C:Spc110	C:Spc42	1.06	0.07	42	BESY38
C:Spc110	C:Spc29	1.32	0.07	81	DHY212
C:Spc110	N:Spc42	2.18	0.22	101	DHY209
C:Spc110	C:Cmd1	2.37	0.21	93	BESY86-12D
Spc110-YFP-CFP	Spc110-YFP-CFP	2.42	0.22	95	DHY150
C:Spc29	N:Spc110	1.03	0.07	107	EMY180
C:Spc29	C:Cnm67	1.1	0.08	70	DHY41
C:Spc29	C:Spc42	1.14	0.07	87	DHY47-6B
C:Spc29	C:Spc110	1.42	0.13	81	DHY38
C:Spc29	C:Cmd1	1.7	0.15	84	BESY89-1C
C:Spc29	N:Spc42	2.24	0.18	72	DHY208
C:Spc42	N:Spc29	0.98	0.09	84	EMY181-1D
C:Spc42	N:Spc110	1.02	0.06	82	EMY179
C:Spc42	C:Spc110	1.08	0.11	42	EMY190
C:Spc42	C:Cmd1	1.13	0.08	99	BESY91-2C
C:Spc42	C:Spc29	1.2	0.14	87	EMY194-2C
C:Spc42	C:Cnm67	2.06	0.24	81	EMY195-9A
N:Spc42	N:Spc110	1.04	0.07	89	BESY25
N:Spc42	C:Cnm67	1.11	0.06	77	BESY22
CFP-Spc42-YFP	CFP-Spc42-YFP	1.25	0.08	49	BESY31
YFP-Spc42-CFP	YFP-Spc42-CFP	1.29	0.09	49	BESY34
N:Spc42	C:Spc110	1.86	0.17	101	BESY23
N:Spc42	C:Spc29	1.86	0.15	72	BESY45
N:Spc42	C:Cmd1	2.1	0.16	90	BESY88-8A

C and N, the position of the tag at the C- or N-terminus, respectively.