Table 2.

Classification of FRET_R values

Row	CFP- and YFP-labeled protein pair	Line segment in proof	FRETR mean	SD	Tukey-Kramer HSD levels	FRET class
1	C:Spc42 and N:Spc29		0.98	0.09	1	None
2	N:Spc110 and C:Cnm67		0.99	0.07	1	Control None
3	C:Cnm67 and N:Spc29		0.99	0.10	1	None
4	N:Spc110 and C:Cmd1		1.01	0.11	1	Control None
5	C:Spc42 and N:Spc110		1.02	0.06	1, 2	Control None
6	N:Spc110 and C:Spc110		1.02	0.06	1	Control None
7	N:Spc110 and C:Spc29		1.03	0.07	1, 2	Control None
8	C:Cnm67 and C:Spc110		1.04	0.06	1, 2	None
9	N:Spc42 and N:Spc110		1.04	0.07	1, 2	Control None
10	C:Spc42 and C:Spc110	AF	1.07	0.09	1, 2, 3	Lowest
11	C:Cnm67 and C:Cmd1	BC	1.09	0.09	2, 3	Lowest
12	C:Spc42 and C:Cmd1	AC	1.10	0.08	2, 3	Lowest
13	C:Cnm67 and C:Spc29	BD	1.10	0.09	2, 3	Lowest
14	N:Spc42 and C:Cnm67	BE	1.13	0.08	3, 4	Lowest
15	C:Spc42 and C:Spc29	AD	1.17	0.12	4	Lowest
16	N:Spc42 and C:Spc42	AE	1.27	0.09	5	Low
17	C:Spc29 and C:Spc110	DF	1.37	0.12	6	Low
18	C:Spc29 and C:Cmd1	DC	1.69	0.15	7	Moderate
19	N:Spc29 and C:Cmd1		1.75	0.23	7	Moderate
20	N:Spc42 and C:Spc110	EF	2.02	0.25	8	High
21	C:Spc29 and N:Spc42	DE	2.05	0.25	8	High
22	C:Cmd1 and N:Spc42	CE	2.07	0.19	8	High
23	C:Cmd1 and C:Spc110	CF	2.15	0.30	9	High
24	C:Spc42 and C:Cnm67	AB	2.29	0.31	10	High
25	Spc110-YFP-CFP		2.42	0.22	11	Control High
26	Cnm67-YFP-CFP		2.65	0.18	12	Control High

The average of the reciprocal pairs in Table 1. The line segments for the geometric proof in Supplementary Materials represent the distances between the listed termini. For the Tukey-Kramer comparison of means, pairs not sharing the same level are significantly different at an alpha of 0.05.