Figure 1.

NF-IL6β production was increased by EGF in A431 cells. (A and B) A431 cells were starved for 6 h and then restimulated with 50 ng/ml EGF. Total RNA or cell lysates were harvested for different time points as indicated for examining by RT-PCR and Western blotting analysis of NF-IL6β mRNA and protein level, respectively. (C) Effect of cycloheximide treatment on the transcription of NF-IL6β mRNAs. The A431 cells were starved for 6 h and then pretreated with cycloheximide for 20 min before addition of EGF. Total RNA was isolated after 1 h of EGF treatment for RT-PCR assay.