Figure 9.
Functional role of p38MAPK and CREB in EGF stimulated NF-IL6β promoter activity in A431 cells. (A) A431 cells were transiently transfected with reporter genes as indicated along with a control (pCDNA3) or the pCDNA3/CREBS133A (DN-CREB) expression vectors. After transfection, cells were cultivated in medium with or without EGF. Cell lysates were then prepared and analyzed for luciferase activity. (B) A431 cells transfected with pGL2-promoter or CRE-heterologous reporter genes combined with the control, p38α, or CREB expression vectors, as indicated, were stimulated with or without EGF for 15 h. Cell lysates were then prepared and analyzed for luciferase activity. (C) Dominant negative forms of p38α or CREB were cotransfected with pGL2NF-IL6β1xCRE reporter in A431 cells. For the normalization, the pGL2NF-IL6β1xCRE reporter activity cotransfected with control vector, pCDNA3, was assigned a value of 100. The reporter activities of pGL2NF-IL6β1xCRE combined with DN-p38α or DN-CREB were normalized to the control. The data shown are means ± standard deviations of two independent experiments.