Figure 8.

Recruitment of MHCIIB by Tm5NM1 to the growth cone. Primary cortical neurons cultured for 5 d were double immunofluorescence stained with MHCIIB (a, d, g, j, and m) and actin (b, e, and k) or LC1 (h) or TM311 to detect Tm3 (n). (c) Merged image of a (red) and b (green). (f) Merged image of d (red) and e (green). (i) Merged imaged of g (red) and h (green). (l) Merged image of j (red) and K (green). (o) Merged image of m (red) and n (green). Note the enrichment of MHCIIB in the peripheral region of the Tm5NM1 growth cones (d and g) where actin (e) and the exogenous Tm5NM1 (h) also are enriched. In contrast, such enrichment of MHCIIB was not observed in control (a) or Tm3 growth cones (j and m). Bar, 8 μm.